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First Steps of in Situ Surface-Enhanced Raman Scattering During Shipboard Experiments ArchiMer
Peron, Olivier; Rinnert, Emmanuel; Colas, Florent; Lehaitre, Michel; Compere, Chantal.
It is shown that the surface-enhanced Raman scattering (SERS) technique can be applied to detect organic molecules during in situ experiments. To this purpose, we used trans-1,2-bis(4-pyridyl)ethylene (BPE) as a target molecule. Adsorbed on the SERS chemosensor surface and excited under laser, the vibration modes of the molecules can be identified. SERS chemosensors are based on quartz substrates functionalized by silanization and partially coated with gold nanoparticles. SERS measurements during shipboard experiments were made with a home-made in situ Raman spectrometer connected to a marinized micro-fluidic system. The device was designed to host chemosensors in order to ensure measurements with a flow cell. A theoretical limit of detection was estimated...
Tipo: Text Palavras-chave: Surface-enhanced Raman scattering; SERS; Chemosensors; Flow cells; Shipboard experiments; In situ measurements; Freundlich isotherm.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00016/12691/9718.pdf
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Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology ArchiMer
Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Thioune, Nene; David, Catalina; De La Chapelle, Marc; Compere, Chantal.
We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with...
Tipo: Text Palavras-chave: Biosensor; Surface-enhanced Raman scattering; Polycyclic aromatic hydrocarbons; Benzo[a]pyrene antibody; Organometallic nanoprobe.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00417/52844/53870.pdf
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